Bromodeoxyuridine-labeled bacteriophage T4D
نویسندگان
چکیده
منابع مشابه
Polynucleotide ligase in bacteriophage T4D recombination.
Following infection of E. coli B with ligase-deficient rII bacteriophage T4D recombination between linked markers is increased 4.2 fold and heterozygote frequency increased 2.3 fold. In such infection recombination occurs at a rapid rate for an extended period. This is in contrast to the time course of recombination observed in wild-type, lysis-inhibited, or lysis-defective (gene t defective) i...
متن کاملFunction of T4D structural dihydrofolate reductase in bacteriophage infection.
Various properties of the bacteriophage structural dihydrofolate reductase (DFR) have been examined to determine its function during phage infection. It has been found that a binding site for reduced nicotinamide adenine dinucleotide phosphate (NADPH), most likely on the DFR present in the phage tail plate, is required for phage viability. Attachment of adenosine diphosphoribose, an analogue of...
متن کاملGenetic mapping of regA mutants of bacteriophage T4D.
SP62, a mutant of bacteriophage T4 shown by Wiberg et al. (1973) to be defective in regulation of T4 protein synthesis, was shown by complementation tests to define a new gene, regA, and by intergenic mapping to lie between genes 43 and 62. The mapping involved crossing SP62 with a quadruple amber mutant defective in genes 42, 43, 62, and 44, selecting all six classes of amber-containing recomb...
متن کاملAmber mutants of bacteriophage T4D: their isolation and genetic characterization.
We have isolated a large number of mutants of bacteriophage T4D that are unable to form plaques on strain B of Escherichia coli, but are able to grow (nearly) normally on some other strains of E. coli, in particular strain CR63. These mutants, designated amber (am), have been characterized by complementation tests, by genetic crosses, and by their response to chemical mutagens. It is concluded ...
متن کاملA map of molecular distances between mutations of bacteriophage T4D.
map of physical distances along the complete T4 DNA molecule has been A reported by MOSIG (1 968). This map was obtained using T4 phage particles, which contain considerably less DNA than a complete genome (petit particles). Each of these particles contains a continuous and probably random permutation of the T4 DNA molecule. MOSIG estimated the ratio of petit particles which carried a particula...
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ژورنال
عنوان ژورنال: Hereditas
سال: 2009
ISSN: 0018-0661
DOI: 10.1111/j.1601-5223.1973.tb01102.x